Figure 2.

Anthranilate and L-tryptophan are substrates of AspA. (A) ATP-[32P]PPi exchange assay of full length AspA; (B) Loading assay of A1-T1-C2 (M1) and C2-A2-T2-CT (M2) with [14C]anthranilate or [14C]tryptophan. M1 activates Ant while M2 activates L-Trp. Assay components are labeled in the figure. Data represent mean values ± s.d.