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In vitro reconstitution of AspA. (A) LC/MS analysis (280 nm) shows purified AspA generates asperlicin C/D and a third product 1 of identical mass from Ant, L-Trp, and ATP; (B) Catalytic turnover number and product partition ratios for 1, asperlicin C and D production. Both asperlicin C and D standards were synthesized from our previous study (Haynes, et al., 2012).
