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. Author manuscript; available in PMC: 2013 Jul 31.
Published in final edited form as: Proteomics. 2012 Apr;12(7):950–959. doi: 10.1002/pmic.201100618

Fig 3.

Fig 3

NanoLC-ESI-MS/MS analysis of COX subunit Vb. The analysis of the doubly charged precursor ion (927.5 m/z) resulted in the identification of pSer2 in the peptide ApSGGGVPTDEEQATGLER with Mascot® scores of up to 82. The CID experiment (A) of the N-terminal peptide of the mature protein produced typical neutral losses of phosphoric acid from the precursor ion and nearly all b-type ions. Together with the y-ions the C-terminal part of the peptide is well defined. In contrast to the CID-fragmentation behavior, the z-ions of the ETD experiment also define the N-terminal part, including the phosphorylation site, with site-specific ions z16(+1H) and z17(+1H).