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. Author manuscript; available in PMC: 2013 Jul 31.
Published in final edited form as: Am J Trop Med Hyg. 2007 Jan;76(1):12–19.

Figure 2.

Figure 2

Plasmodium vivax Duffy binding protein (Pvdbp) plasmids and a plasmid mixture genotyped by the OLA for 14 SNPs. (A and B) Pvdbp plasmids 1088.2 and 1088.5, respectively, genotyped by the OLA method. Sequencing results are shown below the graphs for each plasmid. (C) OLA results for Pvdbp plasmids 1088.2 and 1088.5 mixed at a 2:1 ratio based on absorbence of PCR products at 260 nm. Assays were performed in triplicate; error bars show SE. Salvador-1 alleles are shown with white bars, and other alleles are shown with black bars. The median fluorescent signals generated for each allele were divided by the sum of the signals for both alleles of a particular SNP to generate the allelic ratio. To be considered positive for a particular allele, the allelic ratio must be greater than the cut-off value as described in Materials and Methods.