Table 1.
Ligase detection reaction primers for genotyping P. vivax variants (VK210 and VK247).
| Variant | Primer sequencea | FlexMap™ microsphere set id no.b |
|---|---|---|
| VK210-TAG | 5′ cta caa aca aac aaa cat tat caa GGW GAY AGA GCA GMT GGA CAR CCA GCA 3′ | 28 |
| VK210 conserved | 5′-/5Phos/GGA AAT GGT GCA GGT GGA CAG GCA GCA/3Bio/−3′ | |
| VK247-TAG | 5′ cta act aac aat aat cta act aac GCA AAT GGR GCN GGY AAT CAA CCA GGA 3′ | 80 |
| VK247 conserved | 5′-/5Phos/GCA AAT GGT GCA GGT GGA CAG GCA GCA/3Bio/−3′ |
W= A or T; Y=T or C; M=A or C; R=G or A, N=A, T, C or G.
a
Primers were designed using pvcsp sequence for both subtypes: VK210 (GenBank accession number M11926), VK247 (GenBank accession number M28745). Lowercase nucleotides represent the TAG sequence specific to the FlexMap™ microsphere and are added to the 5′ end of each variant-specfic LDR primer.
b
ID, identification. One hundred unique Luminex microsphere sets are synthesized to exhibit unique fluorescence. Each microsphere set is coupled to different anti-TAG sequences. Anti-TAG sequences are complementary to allele-specific TAG sequences.