AFM height image of model HIV-1 SLB on mica (imaged in buffer, 18° C, ± 1.6 nm height scale). A) 1.8 × 1.6 μm image showing SLB topography before AH peptides were washed from the surface. Height cross-section was taken along the three domains indicated by the position of the dashed line. The three domains labeled in the height cross-section correspond to the numbers labeled on the AFM height image. B) 1.8 × 1.6 μm image showing SLB topography after AH peptides were washed from the surface. C) Height image demonstrating a complete, defect free SLB over a larger, 8 × 8 μm, area.