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. 2013 Jul;3(7):130019. doi: 10.1098/rsob.130019

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Levels of Spo11-oligonucleotides are increased in the recombination-defective dmc1Δ mutant but decreased when combined with the rad24Δ checkpoint mutant. (a) Meiotic recombination is initiated by DSBs created by Spo11 (orange ellipses), which are nucleolytically processed by Mre11, Sae2 and Exo1 generating transient Spo11-oligonucleotide complexes and ssDNA flanking the DSB site. DSB repair uses Dmc1 and Rad51-dependent recombination between homologous chromosomes, which facilitates their pairing and segregation at anaphase I. (b) Spo11-oligonucleotide complexes were immunoprecipitated from whole cell extracts of synchronous cultures of the indicated strains and timepoints after meiotic induction, 3′-labelled with α-³²P dCTP using TdT and resolved by SDS-PAGE. Spo11-oligonucleotides are marked by an arrowhead. Asterisk indicates non-specific TdT labelling. (c) Abundance of Spo11-oligonucleotide complexes was quantified using a phosphorimager and ImageGauge software.