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. 2013 Jul;3(7):130019. doi: 10.1098/rsob.130019

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© 2013 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 5. — Mek1 phosphorylation is synergistically reduced in a checkpoint-defective spo11-HA background. (a,b) Whole cell lysates from the indicated time points and strains were resolved by SDS-PAGE containing 10 µM phos-tag reagent and probed with anti-Mek1 antibody. Mek1 is indicated with an arrowhead; phosphorylated Mek1 by an asterisk. (b) The blot in (ii) is a longer exposure of the blot in (i).