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All titrations were performed at 25°C as described in the Experimental procedures. ITC data were fit to a one-site binding model. ND=not detected.
| Protein in cell | Titrant | N | Kd (μM) | ΔG (kcal mol−1) | ΔH (kcal mol−1) | -TΔS (kcal mol−1) |
|---|---|---|---|---|---|---|
| GmATPS1 | APS | 1.03±0.01 | 0.44±0.02 | -8.66±0.42 | -14.4±0.1 | 5.69 |
| GmATPS1 | PPi | ND | ND | ND | ND | ND |
| GmATPS1•APS | PPi | 0.96±0.01 | 0.30±0.02 | -8.89±0.51 | -15.8±0.1 | 6.92 |
