Table 2. Primers used in this study for the detection of antibiotic resistance determinants.
Targets | Prime name | Sequence 5′-3′ | Reference | |
---|---|---|---|---|
β–lactamases | ||||
IMP–type | ||||
IMP–1, –4, –6 | IMP–1F | GCGTTTATGTTCATACTTCGTTT | This study | |
IMP–1R | TCTATTCCGCCCGTGCTGT | |||
IMP–8 | IMP–8F | AGCGGCTTTGCCTGATT | This study | |
IMP–8R | GACCGTCCGGTTTAACAAA | |||
VIM–type | ||||
VIM–1,–4,–5,–12,–19 | VIM–1F | GACCGCGTCTGTCATGG | This study | |
VIM–1R | GGCGACTGAGCGATTTTT | |||
VIM–2, –23, –24 | VIM–2F | GTCTATTTGACCGCGTCTATCATGGC | This study | |
VIM–2R | CGTTGCGATATGCGACCAAAC | |||
KHM–1 | KHM–FD | AGTGGATTGACGCGCAGGGC | This study | |
KHM–RV | TTCCAGCAGCGATGCGTCGC | |||
NDM–1 | NDM–FD | CAACTGGATCAAGCAGGAGA | This study | |
NDM–RV | TCGATCCCAACGGTGATATT | |||
NDM–1, Full length | NDM-FF | ATGGAATTGCCCAATATTATG | This study | |
NDM-RR | TCAGCGCAGCTTGTCGGCC | |||
KPC–type | KPC–1F | CGTTGACGCCCAATCC | This study | |
KPC–1R | ACCGCTGGCAGCTGG | |||
GES–type | GES–7F | ATCTTGAGAAGCTAGAGCGCG | This study | |
GES–1 to 5 and –7 | GES–7R | GTTTCCGATCAGCCACCTCT | ||
DHA–1 | DHA–F | GTCGCGGCGGTGGTGGAC | This study | |
DHA–R | CCGCACCCAGCACACCTGT | |||
CMY–1–type | CY1–GC1M–F | GCTGCTCAAGGAGCACAGGATCCCG | 7 | |
CY1–GC1M–R | GGCACATTGACATAGGTGTGGTGCATG | |||
CMY–2–type | CY–GC2M–F | ACTGGCCAGAACTGACAGGCAAA | 7 | |
CY–GC2M–R | GTTTTCTCCTGAACGTGGCTGGC | |||
OXA–48 | OXA–48F | ATGCGTGTATTAGCCTTATCGGC | This study | |
OXA–48R | ACTTCTTTTGTGATGGCTTGGCGCA | |||
TEM–type | TEM–F | ATAAAATTCTTGAAGACGAAA | 8 | |
TEM–R | GACAGTTACCAATGCTTAATCA | |||
SHV–type | SHV–F | GGGTTATTCTTATTTGTCGC | 8 | |
SHV–R | TTAGCGTTGCCAGTGCTC | |||
CTX–type | CTX–F | AAAAATGATTGAAAGGTGGTTGT | 8 | |
CTX–R | TTACAGCCCTTCGGCGATGA | |||
16S rRNA methylases | ||||
rmtA | RMTA–F | TTCCCTCTGCCCGGATACCG | This study | |
RMTA–R | CAACCCCTGATGGATGTCG | |||
rmtB | RMTB–F3 | GAGCTGGATACCCTGTACGA | This study | |
RMTB–R4 | GGCAAAGGTAAAATCCCAAT | |||
rmtC | RMTC–F | TATGGTGCTTATATTGGTGGG | This study | |
RMTC–R | GCATGCCAGCCTCCGTAAAG | |||
rmtD | RMTD–F | GCACGTGCGCCTCCATCCATTC | This study | |
RMTD–R | CGTTTGGGCGATTTGCTGTGCG | |||
armA | ARMA–F1 | ATTCTGCCTATCCTAATTGG | 9 | |
ARMA–R2 | ACCTATACTTTATCGTCGTC | |||
npmA | NPMA–F1 | TTTGGGTACTGGAGACGGTA | This study | |
NPMA–R2 | TCAATGCGAAAACCTGAGTT |