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. 2012 Mar 29;3(1):19–24. doi: 10.5365/WPSAR.2011.2.4.010

Table 2. Primers used in this study for the detection of antibiotic resistance determinants.

Targets Prime name Sequence 5′-3′ Reference
β–lactamases
IMP–type
IMP–1, –4, –6 IMP–1F GCGTTTATGTTCATACTTCGTTT This study
IMP–1R TCTATTCCGCCCGTGCTGT
IMP–8 IMP–8F AGCGGCTTTGCCTGATT This study
IMP–8R GACCGTCCGGTTTAACAAA
VIM–type
VIM–1,–4,–5,–12,–19 VIM–1F GACCGCGTCTGTCATGG This study
VIM–1R GGCGACTGAGCGATTTTT
VIM–2, –23, –24 VIM–2F GTCTATTTGACCGCGTCTATCATGGC This study
VIM–2R CGTTGCGATATGCGACCAAAC
KHM–1 KHM–FD AGTGGATTGACGCGCAGGGC This study
KHM–RV TTCCAGCAGCGATGCGTCGC
NDM–1 NDM–FD CAACTGGATCAAGCAGGAGA This study
NDM–RV TCGATCCCAACGGTGATATT
NDM–1, Full length NDM-FF ATGGAATTGCCCAATATTATG This study
NDM-RR TCAGCGCAGCTTGTCGGCC
KPC–type KPC–1F CGTTGACGCCCAATCC This study
KPC–1R ACCGCTGGCAGCTGG
GES–type GES–7F ATCTTGAGAAGCTAGAGCGCG This study
GES–1 to 5 and –7 GES–7R GTTTCCGATCAGCCACCTCT
DHA–1 DHA–F GTCGCGGCGGTGGTGGAC This study
DHA–R CCGCACCCAGCACACCTGT
CMY–1–type CY1–GC1M–F GCTGCTCAAGGAGCACAGGATCCCG 7
CY1–GC1M–R GGCACATTGACATAGGTGTGGTGCATG
CMY–2–type CY–GC2M–F ACTGGCCAGAACTGACAGGCAAA 7
CY–GC2M–R GTTTTCTCCTGAACGTGGCTGGC
OXA–48 OXA–48F ATGCGTGTATTAGCCTTATCGGC This study
OXA–48R ACTTCTTTTGTGATGGCTTGGCGCA
TEM–type TEM–F ATAAAATTCTTGAAGACGAAA 8
TEM–R GACAGTTACCAATGCTTAATCA
SHV–type SHV–F GGGTTATTCTTATTTGTCGC 8
SHV–R TTAGCGTTGCCAGTGCTC
CTX–type CTX–F AAAAATGATTGAAAGGTGGTTGT 8
CTX–R TTACAGCCCTTCGGCGATGA
16S rRNA methylases
rmtA RMTA–F TTCCCTCTGCCCGGATACCG This study
RMTA–R CAACCCCTGATGGATGTCG
rmtB RMTB–F3 GAGCTGGATACCCTGTACGA This study
RMTB–R4 GGCAAAGGTAAAATCCCAAT
rmtC RMTC–F TATGGTGCTTATATTGGTGGG This study
RMTC–R GCATGCCAGCCTCCGTAAAG
rmtD RMTD–F GCACGTGCGCCTCCATCCATTC This study
RMTD–R CGTTTGGGCGATTTGCTGTGCG
armA ARMA–F1 ATTCTGCCTATCCTAATTGG 9
ARMA–R2 ACCTATACTTTATCGTCGTC
npmA NPMA–F1 TTTGGGTACTGGAGACGGTA This study
NPMA–R2 TCAATGCGAAAACCTGAGTT