Fig. 2.

PCR analysis of mGlu1h mRNA expression in human melanocytes, melanoma, and brain. A unique set of primers specific for mGlu1h cDNA (Table 1) amplified a single, specific band of 989 bp and reveals mRNA of this splice variant is present in melanocytes and may be upregulated in both melanoma cell lines used in this work. mGlu1h message was not detected in an adult human cDNA library. Empty vector was used as a negative control (- control) while vector containing the mGlu1h receptor coding sequence was used as a positive control (+ control). A primer pair to amplify a region of GAPDH (Table 1) was detected at 203 bp, and used as a loading control.