Table 1. PCR Primers used in this study.
Primer Name | Direction | Sequence (5′-3′) | Position | Exon |
---|---|---|---|---|
Adaptor | Reverse | Oligo dT Adaptor Primer | Poly-A tail | N/A |
hmGlu1-2162F | Forward | TTGTGACTTGGGATGGTGGC | 2162-2181a | VII |
hmGlu1-2328F | Forward | ACACCAGTGGTCAAATCCTCCAG | 2328-2350a | VIII |
hmGlu1-2661F | Forward | ATGCCCATTCTGTCCTACCCAAGT | 2661-2684a | VIII |
hmGlu1-3066F | Forward | CCCTGCCGCTCCAACACTTTCCTCA | 3066-3090a | VIII |
hmGlu1-3126R | Reverse | GCCAAGCCAAGAAGAATCCTAACTCCCAAG | 3130-3126a 390077-390058b |
VIII to IXa overlap |
hmGlu1-3286R | Reverse | GAAAAGGTCAGGCTCTTGCCAGAGC 3310-3286a | 3310-3286a | X |
hmGlu1g-R | Reverse | AGGTCCCATGCGAAAGGGTAAGTT | 377377-377354b | VIII-extended |
hmGlu1h-R | Reverse | TCTTGGGAGTTAGGATTCTTC | 390078-390058b | IXb |
GAPDH F | Forward | CCACCCAGAAGACTGTGGAT | 722-741c | N/A |
GAPDH R | Reverse | ACCTGACCTGCCGTCTAGAA | 924-905c | N/A |
Position refers to the sequence in GenBank ID: NM_001114329.1
Position refers to the sequence in GenBank ID: NT_025741.15
Position refers to the sequence in GenBank ID: NM_002046.4