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. Author manuscript; available in PMC: 2014 Jun 20.
Published in final edited form as: Cell. 2013 Jun 20;153(7):1510–1525. doi: 10.1016/j.cell.2013.05.021

Figure 6. LKB1 mediates axonal branching through mitochondria immobilization along the axon.

Figure 6

(A–E) Representative kymographs of axonal mitochondria in dissociated neurons electroporated with the indicated constructs. (F–J) Representative neurons at 5 DIV electroporated with the constructs indicated in A–E. Red arrowheads point to axon branches. (K) Loss of LKB1 caused a decrease in the percentage of stationary mitochondria, while overexpression of wildtype human SNPH upon loss of LKB1 returned mitochondrial transport to normal levels. (L) Loss of LKB1 decreased the number of axonal branches, while overexpression of wildtype human SNPH upon loss of LKB1 returned axonal branching to normal levels. (M) Overexpression of LKB1 increased the percentage of stationary mitochondria, while overexpression of dominate negative human SNPH-ΔMTB at the same time as LKB1 returned mitochondrial transport to normal levels. (N) Overexpression of LKB1 also increased the number of axonal branches, while overexpression of dominate negative human SNPH-ΔMTB at the same time as LKB1 returned axonal branch number to normal levels. Data represent average value ±SEM (K–N). Statistical analysis: ANOVA non-parametric test.