Micro-coil pulsed field gradient-stimulated echo (PFG-STE) NMR measurements of translational diffusion coefficients, Dt, of the β2AR-T4L–timolol complex in aqueous DDM solutions, pH = 7.5, T = 298K. Measurements of Dt performed at discrete DDM concentrations are plotted versus the DDM concentration. (A) 0.5 mM β2AR-T4L–timolol complex reconstituted in DDM micelles. (■) Dt,pm obtained from direct 1H NMR observation of the protein (◆)
obtained from 1H NMR observation of DDM. The straight line represents a regression of the Dt,pm values. (B) 0.5 mM β2AR-T4L–timolol complex reconstituted in DDM micelles containing CHS at a CHS:DDM ratio of 0.2. Same presentation as in (A).