Figure 4.

The ectodomain inhibits ERK1/2 phosphorylation in a dose dependent-manner but does not affect the activation kinetics. A: RWPE-1 cells were transferred to basal KSF medium for 30 minutes before the medium was replaced with different amounts of ectodomain containing conditioned medium. Whole cell lysates were then subjected to immunoblotting with anti-p-ERK1/2 and anti-ERK1/2 antibodies for normalization. B: RWPE-1 cells were transferred to basal KSF medium for 30 minutes before the medium was replaced with ectodomain containing conditioned medium for indicated times. Whole cell lysates were prepared and subjected to immunoblotting with anti-p-ERK1/2 and anti-β-tubulin antibodies for normalization. Representative examples of at least two independent experiments showing similar results are shown.