Figure 5.

The TMEFF2 ectodomain promotes AKT phosphorylation and that inversely correlates with its effect on ERK phosphorylation. RWPE-1 cells transferred to basal KSF medium for 4 hours before the medium was replaced with ectodomain containing conditioned medium obtained from HEK293T cells that express the ectodomain (ECTO), the full length (FULL) or the empty vector (EV) constructs. Whole cell lysates were prepared and subjected to immunoblotting with anti-p-ERK1/2, anti-ERK1/2, anti-p-AKT S473, anti-AKT, and anti-p-smad2 antibodies (left). Quantitation of the results is shown (right). Representative examples of at least two independent experiments showing similar results are shown.