Table S3.
Nutritional variables, and plasma concentration of L-Trp and 5-HT and tissue concentrations of L-Trp, 5-HT, and 5-HIAA in the liver, small intestine and regions of brain in WT mice fed the 0.17% L-Trp diet (control), 0.08% L-Trp diet and 0.06% L-Trp diet.
| WT mice | |||
|---|---|---|---|
|
|
|||
| 0.17% L-Trp (control) | 0.08% L-Trp | 0.06% L-Trp | |
| Initial body weight (g) | 21.1 ± 0.5 | 21.0 ± 0.8 | 20.9 ± 0.5 |
| Final body weight (g) | 25.9 ± 0.1 | 26.6 ± 0.7 | 25.9 ± 0.5 |
| Gain in body weight (g/28 d) | 4.7 ± 0.5 | 5.6 ± 0.5 | 5.0 ± 0.3 |
| Total food intake (g/28 d) | 108.8 ± 1.6 | 107.2 ± 2.0 | 108.0 ± 1.6 |
| Liver (g/100 g body weight) | 4.98 ± 0.25 | 5.02 ± 0.09 | 4.77 ± 0.14 |
| Small intestine (g/100 g body weight) | 2.92 ± 0.10b | 3.25 ± 0.10b | 3.98 ± 0.26a |
| Hippocampus (mg/100 g body weight) | 122 ± 5a,b | 110 ± 4b | 126 ± 5a |
| Striatum (mg/100 g body weight) | 89.2 ± 11.2a | 92.9 ± 7.3a | 70.2 ± 5.5b |
| Cerebral cortex (mg/100 g body weight) | 665 ± 25 | 604 ± 16 | 658 ± 11 |
| Plasma | |||
| Trp (nmol/mL) | 76.3 ± 16.6 | 69.0 ± 3.9 | 64.6 ± 9.4 |
| 5-HT (nmol/mL) | 3.24 ± 0.64 | 3.93 ± 0.84 | 3.38 ± 0.36 |
| Hippocampus | |||
| Trp (nmol/g of tissue) | 63.0 ± 1.59a | 68.0 ± 3.71a | 42.8 ± 5.59b |
| 5-HT (nmol/g of tissue) | 5.78 ± 0.13 | 5.96 ± 0.11 | 4.68 ± 0.54 |
| 5-HIAA (nmol/g of tissue) | 3.67 ± 0.15a | 3.64 ± 0.23a | 2.18 ± 0.45b |
| Striatum | |||
| Trp (nmol/g of tissue) | 20.6 ± 0.80 | 18.7 ± 1.4 | 15.7 ± 1.9 |
| 5-HT (nmol/g of tissue) | 4.76 ± 0.49 | 4.24 ± 0.41 | 4.06 ± 0.25 |
| 5-HIAA (nmol/g of tissue) | 2.53 ± 0.21a | 2.03 ± 0.26a,b | 1.57 ± 0.20b |
| Cerebral cortex | |||
| Trp (nmol/g of tissue) | 65.1 ± 3.47 | 62.9 ± 2.80 | 53.5 ± 5.72 |
| 5-HT (nmol/g of tissue) | 3.81 ± 0.07 | 3.78 ± 0.09 | 3.92 ± 0.34 |
| 5-HIAA (nmol/g of tissue) | 1.82 ± 0.08a | 1.51 ± 0.07a,b | 1.22 ± 0.17b |
| Liver | |||
| Trp (nmol/g of tissue) | 38.9 ± 3.1a,b | 39.3 ± 1.1a | 28.4 ± 3.1b |
| Small intestine | |||
| 5-HT (nmol/g of tissue) | 56.9 ± 2.2 | 53.2 ± 6.0 | 31.8 ± 7.3 |
| Urine | |||
| Trp (nmol/day) | 33.0 ± 1.2 | 30.4 ± 5.1 | 24.4 ± 6.8 |
| 5-HT (nmol/day) | ND | ND | ND |
| 5-HIAA (nmol/day) | 47.9 ± 1.7a | 39.3 ± 3.5a,b | 30.2 ± 2.4b |
Notes: Values are the mean ± SEM, n = 3–5. The means in a row with superscript letters without a common letter differ, P < 0.05, as determined by oneway ANOVA and was followed by Tukey’s multiple-comparison test.