NA-TLRs are required for development of lupus nephritis in MRL-Faslpr mice. (A) Protein concentration in urine (left panel) and GN scores (right panel) from 7- to 8-mo-old 3d and WT/Het MRL-Faslpr. Urine protein in female B6 mice was 2.5 ± 0.1 mg/ml (n = 4). (B) Representative periodic acid-Schiff–stained kidney sections of 7- to 8-mo-old WT/Het and 3d mice. Glomerular enlargement, deposits (indicated by arrow), and proliferative changes in the mesangium of WT, but not in 3d kidneys, can be observed. (C) IgG immunofluorescence staining and quantification of frozen kidney sections. Fluorescent intensity was normalized to glomerular area. Seven- to 8-mo-old WT/Het and 3d (6–13 mice/group). (D) Kaplan-Meier survival plot of F10 WT and 3d MRL-Faslpr mice (6–11 mice/group). Data representative of three independent experiments for (A)–(C). Survival study was carried out once. +p < 0.05, ++
p < 0.01, +++p < 0.001.