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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Calcif Tissue Int. 2012 Aug 4;91(3):215–224. doi: 10.1007/s00223-012-9628-z

Figure 4. Deletion of Cx43 results in decreased expression of Lox.

Figure 4

(A) mRNA levels of the indicated genes in calvaria cells isolated from the progeny of DMP-GFP;DMP1-8kb-Cre separated by FACS in GFP-negative and GFP-positive fractions. Mrsp2 was used as housekeeping gene. OB: osteoblasts; OT: osteocytes. (B) mRNA levels of the indicated genes in RNA purified from tibia diaphysis from wild type C57BL/6 mice. Mrsp2 was used as housekeeping gene. Bars are mean ± SD. *, indicates significant differences at p<0.05 vs Lox levels, n = 6. (C) Expression levels of Cx43, Lox, and Loxl-1 in primary calvaria cells from Cx43fl/− and OCN-Cre;Cx43fl/− mice and Cx43fl/fl and DMP1-8kb-Cre;Cx43fl/fl mice after 7 days in culture with ascorbic acid to induce differentiation into the osteogenic lineage. Data are expressed as relative to Mrsp2. Bars are mean ± SD. n.s. indicates not significant. (D) Expression levels of Cx43, Lox, and Loxl-1 in osteoblastic OB-6 cells and osteocytic MLO-Y4 cells with (scr shRNA) and without Cx43 (Cx43 shRNA). Data are expressed as relative to Mrsp2. Bars are mean ± SD. *, indicates significant differences at p<0.05, n = 3.