Skip to main content
. Author manuscript; available in PMC: 2013 Jul 31.
Published in final edited form as: Gastroenterology. 2012 Apr 1;143(1):145–154. doi: 10.1053/j.gastro.2012.03.042

Figure 2.

Figure 2

KO Tregs are unable to inhibit proliferation of WT CD4+CD45RBhi cells in Rag1−/− recipients. Rag1−/− mice were adoptively transferred with 4 × 105 CD4+CD45RBhi (Teff) and 1 × 105 CD4+CD45RBloCD25+ (Treg) cells from WT or KO mice. (A) BrdU incorporation in CD4+ splenocytes was determined by flow cytometry 2 weeks after the transfer. (B) BrdU incorporation (brown color) was detected in epithelial cells (blue arrows) and cells infiltrating the colon lamina propria (red arrows) of recipients that were cotransferred with KO Tregs (scale bar, 20 mm; original magnification 200×).