Table 2.
Detailed gene expression analysis of responsive genes
| Gene symbol | Gene alias | Gene function | Saline/0 μg NP | Saline/400 μg NP | OVA/0 μg NP | OVA/400 μg NP |
|---|---|---|---|---|---|---|
|
Cxcl1 |
KC |
Neutrophil chemokine |
1.00+/-0.05 |
2.80+/-0.78a |
2.48+/-0.28b |
6.29+/-0.38a,b |
|
Cxcl2 |
MIP-2 |
PMN chemokine |
1.00+/-0.08 |
2.85+/-0.75a |
4.22+/-0.76b |
8.77+/-0.99a,b |
|
Cxcl10 |
IP10 |
T-cell chemokine |
1.00+/-0.05 |
1.17+/-0.05 |
14.32+/-3.95b |
44.84+/-4.29a,b |
|
Ccl2 |
MCP-1 |
Monocyte and T-cell chemokine |
1.00+/-0.08 |
1.50+/-0.28 |
3.66+/-0.86b |
13.84+/-1.71a,b |
|
Ccl8 |
MCP-2 |
Monocyte chemokine |
1.00+/-0.08 |
1.56+/-0.25 |
15.48+/-5.22b |
105.12+/-32.47a,b |
|
Ccl11 |
Eotaxin |
Eosinophil chemokine |
1.00+/-0.05 |
1.32+/-0.11 |
2.50+/-0.71 |
11.47+/-2.84a,b |
|
Itln1 |
Itlna |
Pathogen-associated molecular pattern recognition |
1.00+/-0.25 |
4.95+/-1.78a |
6.14+/-0.95b |
38.65+/-13.92a,b |
|
Irg1 |
Irg1 |
Toll like receptor response |
1.00+/-0.14 |
4.70+/-1.15a |
15.45+/-4.36b |
154.18+/-16.97a,b |
|
Chi3l3 |
YM1 |
Chitinase-like protein |
1.00+/-0.07 |
1.01+/-0.12 |
1.30+/-0.26 |
1.94+/-0.28b |
|
Chi3l4 |
YM2 |
Chitinase-like protein |
1.00+/-0.35 |
4.61+/-2.98 |
9.13+/-3.96 |
33.10+/-12.56b |
|
Saa3 |
Saa3 |
Acute phase protein |
1.00+/-0.09 |
4.51+/-1.85 |
65.9+/-12.7b |
157.88+/-19.38a,b |
|
Tnfα |
TNFα |
Acute phase response cytokine |
1.00+/-0.12 |
1.69+/-0.17a |
2.20+/-0.23b |
3.36+/-0.30b |
|
Il6 |
IL6 |
Acute phase response cytokine |
1.00+/-0.13 |
-1.14+/-0.13 |
2.00+/-0.40b |
5.60+/-0.96a,b |
|
Il1β |
IL1β |
Acute phase response cytokine |
1.00+/-0.13 |
1.01+/-0.05 |
1.26+/-0.03 |
1.71+/-0.13b |
|
Il1rn |
IL1rn |
IL1 receptor antagonist |
1.00+/-0.04 |
1.13+/-0.07 |
1.70+/-0.23 |
4.62+/-0.71a,b |
|
Il2 |
IL2 |
Th1 cytokine |
1.00+/-0.06 |
1.06+/-0.07 |
1.57+/-0.24b |
1.37+/-0.09 |
|
Ifnγ |
IFNγ |
Th1 cytokine |
1.00+/-0.08 |
-1.06+/-0.14 |
1.67+/-0.28b |
2.31+/-0.30b |
|
Il4 |
IL4 |
Th2 cytokine |
1.00+/-0.15 |
1.01+/-0.12 |
1.40+/-0.26 |
3.77+/-0.77a,b |
|
Il5 |
IL5 |
Th2 cytokine |
1.00+/-0.10 |
-1.20+/-0.18 |
2.39+/-0.59 |
7.17+/-1.44a,b |
|
Il13 |
IL13 |
Th2 cytokine |
1.00+/-0.10 |
1.34+/-0.39 |
6.80+/-2.96b |
44.23+/-12.67a,b |
|
Retnla |
Fizz 1, HIMF |
Th2 suppressor |
1.00+/-0.12 |
1.32+/-0.37 |
6.88+/-1.78b |
21.90+/-3.98a,b |
|
Il17a |
IL17A |
Th17 cytokine |
1.00+/-0.16 |
2.52+/-0.69 |
8.34+/-2.32b |
46.24+/-6.02a,b |
|
Foxp3 |
Foxp3 |
T-reg transcription factor |
1.00+/-0.08 |
1.49+/-0.18 |
2.08+/-0.19b |
3.98+/-0.33a,b |
|
Il10 |
IL10 |
T-reg cytokine |
1.00+/-0.09 |
1.09+/-0.17 |
2.97+/-0.76b |
8.22+/-2.01a,b |
|
Muc5ac |
Muc5ac |
Mucin production |
1.00+/-0.19 |
1.61+/-0.61 |
4.72+/-1.44b |
25.97+/-4.70a,b |
|
Clca3 |
Gob5 |
Ion channel, mucus secretion |
1.00+/-0.39 |
2.52+/-1.42 |
6.16+/-2.26 |
16.72+/-3.42b |
|
Clca2 |
Clca2 |
Ion channel, fibrosis development |
1.00+/-0.12 |
1.40+/-0.16 |
3.25+/-0.78b |
7.96+/-1.20a,b |
|
Mmp12 |
Mmp12 |
Macrophage elastase |
1.00+/-0.08 |
3.06+/-0.81 |
1.83+/-0.22 |
7.91+/-0.59 |
| Timp1 | Timp1 | Mmp inhibitor | 1.00+/-0.05 | -1.00+/-0.10 | 3.73+/-0.87b | 12.40+/-1.68a,b |
A qPCR analysis was performed on the genes that showed a ≥2 fold increase towards untreated controls in the gene array. a: Significant changes (p < 0.05) towards non-SNP exposed animals, b: significant changes towards non-OVA mice. The analysis reveals that neutrophil chemokines (Kc, Cxcl1; Mip-2, Cxcl2) and innate immune responsive genes (Tnfα; Irg1; Itln1) are significantly elevated by SNP exposure in both, SAL- and OVA-mice. A significant adjuvant SNP effect in SNP/OVA-mice was measured for Th2 cytokines (Il4; Il5; Il3) and Th17 associated genes (Il17a; Il6; Saa3). Monocyte and eosinophil chemokines (Mcp-1; Mcp-2; Eotaxin; Ip10) were further significantly increased in SNP/OVA-mice as well as other proteins which might be involved in tissue remodeling (Fizz1; Timp1). No adjuvant SNP effect was measured in Th1 cytokines (Ifnγ; Il2).