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. Author manuscript; available in PMC: 2013 Jul 31.
Published in final edited form as: Science. 2011 Jul 29;333(6042):637–642. doi: 10.1126/science.1205295

Fig. 1.

Fig. 1

Cell-selective Di expression via RC∷FPDi. (A) This Gt (ROSA)26Sor knock-in allele consists of CAG regulatory elements, an FRT-flanked transcriptional Stop, a loxP-flanked mCherry-Stop, and HA-tagged-Di-encoding sequence. A hypothetical example (left) illustrates intersectional restriction of Di to a serotonergic neuron subset (gray area, lower schema) after Flpe- and Cre-recombination; mCherry marks Flpe-only cells (red areas represent serotonergic nuclei); neither Di nor mCherry are expressed in Cre-only cells (yellow circumscribed area). (B) Derivative Di alleles. (C, D) In RC∷PDi, RC∷rePe, Slc6a4-cre brainstems, we observed concurrent reproducible expression of HA-tagged-Di (far-red indirect HA-immunofluorescence), eGFP, and Tph2 (indirect immunofluorescence). DAPI, nuclear stain 4’,6-diamidino-2-phenylindole.