Table 1.
Physiological Parameters and Cardiovascular Ultrasound Study of 6- and 24-Month-Old Eln+/+ and Eln+/− Mice. In Each Group, n = 12–13 for Body and Heart Weights and n = 4 for Mean Arterial Pressure
| 6 month-old mice | 24 month-old mice | |||
|---|---|---|---|---|
| Eln+/+ | Eln+/− | Eln+/+ | Eln+/− | |
| BW(g) | 36.0 ± 1.1 | 35.3 ± 1.3 | 35.3 ± 0.8 | 33.0 ±1.0 |
| Heart weight/BW (%) | 0.43 ± 0.01 | 0.50 ± 0.02G | 0.53 ± 0.03A | 0.62 ± 0.02G |
| Left ventricle/BW (%) | 0.35 ± 0.01 | 0.40 ± 0.02G | 0.43 ± 0.02A | 0.49 ± 0.02G |
| Mean arterial pressure (mmHg) | 103 ± 11 | 123 ± 2G | 107 ± 4 | 138 ± 8G |
| Ultrasound study: | ||||
| Number of animals | 5 | 4 | 10 | 9 |
| Systolic aorta diameter (mm) | 1.95 ± 0.08 | 1.68 ± 0.10G | 2.24 ± 0.15 | 1.85 ± 0.13G |
| Diastolic aorta diameter (mm) | 1.8 ± 0.07 | 1.54 ± 0.10G | 2.12 ± 0.15 | 1.75 ± 0.13G |
| Heart rate (beats per minute) | 504 ± 11 | 499 ± 3 | 508 ± 16 | 496 ± 17 |
| LVEDD/BW (mm) | 0.11 ± 0.002 | 0.12 ± 0.01 | 0.12 ± 0.01 | 0.13 ± 0.01 |
| Left atrium dimension-La (mm) | 3.14 ± 0.05 | 3.17 ± 0.14 | 3.26 ± 0.20 | 3.00 + 0.14 |
| Shortening fraction-FS (%) | 41 ± 3 | 41 ± 2 | 40 ± 5 | 39 + 3 |
| Vcfc (circ/s) | 2.80 ± 0.20 | 2.70 ± 0.20 | 3.01 ± 0.29 | 3.00 ± 0.16 |
| Sa (cm/s) | 3.5 ± 0.3 | 3.1 ± 0.2 | 2.8 ± 0.4 | 2.7 ± 0.3 |
| Spw (cm/s) | 3.80 ± 0.10 | 3.60 ± 0.30 | 3.60 ± 0.20 | 2.97 ± 0.40 |
| Isovolumic relaxation time (ms) | 15.0 ± 0.5 | 17.0 ± 3.0 | 19.5 ± 0.7A | 17.5 ± 1.5 |
| Ea (cm/s) | 4.3 ± 0.3 | 5.3 ± 0.5 | 4.1 ± 0.3 | 5.3 ± 0.4 |
| Epw (cm/s) | 4.1 ± 0.3 | 4.6 ± 0.2 | 5.0 ± 0.6 | 4.1 ± 0.4 |
| E/Ea | 6.0 | 20.0 ± 3.0 | 25.0 ± 2.3 | 19.0 ± 2.1 |
A
Significant difference with adult animals of the same genotype.
G
Significant difference with Eln+/+ animals of matching age.
BW, body weight; LVEDD, left ventricle end diastolic diameter; Vcfc, mean shortening velocity of circumferential fibers, corrected for time; Sa, Spw: maximal systolic velocity of the mitral annulus and posterior wall, respectively. Ea, Epw: maximal diastolic velocity of the mitral annulus and posterior wall, respectively. E/Ea: maximal velocity of LV mitral inflow to Ea.