Figure 2. Comparison of ADCC activity between MX35 and Rebmab200 (stable pool) over a range of mAb concentrations.

The % of cytotoxicity represents antibody-mediated cell lysis measured by release of ⁵¹Cr from labeled ovarian cancer cells (OVCAR-3). Effector cells were obtained from donated human peripheral blood. Rebmab100 was used as a positive control, and Zenapax (Roche) was used as a negative control. The assay was repeated with MCF-7 cells, a NaPi2b negative and Le^Y positive (Rebmab100 antigen) tumor cell line, showing no ADCC results (data not shown).