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. 2013 Jul 31;8(7):e69812. doi: 10.1371/journal.pone.0069812

Figure 2. Comparison of prenatal human cardiac progenitor cells transfected using oscillating magnet array-based nanomagnetic transfection.

Figure 2

(a) Fluorescent microscope images of prenatal human cardiac progenitor cells transfected with pEGFP-N1 representing each conditions, took 48 h post transfection. (I) GFP fluorescence and Hoechst image for 2 Hz 0.2 mm, (II) GFP fluorescence and Hoechst image for 4 Hz 0.2 mm, (III) GFP fluorescence and Hoechst image for static magnetofection™, (IV) GFP fluorescence and Hoechst image for electroporation (V) GFP fluorescence and Hoechst image for lipid based transfection reagent, (VI) GFP fluorescence and Hoechst image for un-transfected (b) Prenatal human cardiac progenitor cells were transfected with pEGFP-N1 as indicated, and scored using manual counting using ImageJ software, 48 h after transfection. Data shown are the media ± SD. n = 12 for 4 Hz 0.2 mm. n = 9 for 2 Hz 0.2 mm and static conditions. n = 6 for 4 Hz 0.4 mm, 4 Hz 0.6 mm, no magnet, electroporation and pEGFP-N1 only electroporation conditions. n = 3 for 2 Hz 0.4 mm, 2 Hz 0.6 mm, pEGFP-N1 only, Neuromag only, and un-transfected conditions. (*p<0.05, ***p<0.001 - Statistically significant).