Figure 5. Sst cells inhibit all other categories but one another.
a) Schematic of experimental configuration: ChR2 expressing Sst-Cre cells are photo-stimulated while recording from a pyramidal cell (Pyr) and a neighboring GAD65/67 positive inhibitory neuron expressing GFP.
b) Example IPSCs simultaneously recorded in the reference pyramid (black) and in one of the six different interneuron categories (different colors). The order of the six pyramid IPSCs (top to bottom) matches the order of the IPSC simultaneously recorded in each of the six interneuron categories. For simplicity, all traces were scaled such that the pyramid IPSCs have the same peak amplitude.
c–h) Left panels: The inhibitory postsynaptic charge (IPSQ) evoked by Sst cell photostimulation and recorded in individual interneurons (IPSQIN; y-axis) is plotted against the IPSQ simultaneously recorded in a pyramidal cell (IPSQPyr; x-axis; see (a) for symbol legend). Dotted line is unity line. Note that all inhibitory neuron categories receive inhibition comparable to that simultaneously recorded in pyramids, but for Sst cells that receive none (d). Category(n of cells/slices/mice): Pvalb(13/10/5), Sst(12/6/5), VIP(10/7/6), Tnfaip8l3(13/10/6), Undefined-UD(16/13/6), L1(8/6/5).
i) Panel showing mean±s.e.m of individual neuronal contributions (INC) of all recorded pairs of the respective category.
j) Schematic illustration of the inhibition mediated by Sst cells onto each interneuron category (abbreviation as in Fig. 2e).