Fig 2.

3D agarose matrix array show basal motility of cells in 3D gel depends on the matrix density. A, Dictyostelium cells migrate in 3D matrixes of agarose of different concentrations (a, 0.2%, b, 0.3%; c, 0.5%; d, in 2D). The initial position of each cell was marked with a number. B, Dcityostelium cells in 3D culture migrated slower than that in 2D culture. Cell motility in 3D matrix is significantly affected by density of the matrix. C, migration persistence in 3D is significantly lower than that of those in 2D culture, more significant in higher density of the agarose. Scale bar = 10 μm. Average of 30-45 cells from 3 independent experiments. (*: P0.01 when compared to that on 2D; #: P<0.01 when compared to that in 0.2% and 0.3% agarose)