Figure 2.
Proposed steps in the NE budding-mediated mRNA export pathway based on [14]. C, cytoplasm; INM, inner nuclear membrane; N, nucleus; ONM, outer nuclear membrane. (a) Formation of intranuclear granules (grey shading) containing endogenous mRNPs is observed, and local disruption of the nuclear lamins is initiated via activity of atypical PKC (aPKC). (b) Changes in the nuclear lamina allow for positioning of the mRNP granule at the INM (i). The INM invaginates to envelope the mRNP granule (ii). Membrane-bound mRNP granule localizes to the perinuclear space between the ONM and INM (iii). The mRNP granule membrane fuses with ONM (iv). (c) The mRNP granule is released into the cytoplasm.
