Table III.
Twenty differentially expressed homeobox containing genes in E13.5 trisomic vs normal embryos.
| Gene | Fold Change (microarray) | p-value | Fold change (qPCR) |
|---|---|---|---|
| Hoxa4 | 0.8331 | 1.50E-05 | N/A |
| Hoxa5 | 0.5501 | 0 | 0.5217 (0.0954) |
| Hoxa7 | 0.7460 | 4.26E-14 | 0.3045 (0.0912) |
| Hoxb2 | 0.8402 | 7.14E-06 | N/A |
| Hoxb4 | 0.6897 | 0 | 0.5613 (0.0404) |
| Hoxb5 | 0.6412 | 0 | 0.4487 (0.1032) |
| Hoxb6 | 0.6895 | 0 | 0.5758 (0.0778) |
| Hoxb7 | 0.5905 | 0 | 0.5890 (0.0853) |
| Hoxb9 | 0.8332 | 1.52E-05 | N/A |
| Hoxc6 | 0.5401 | 0 | 0.3704 (0.1050) |
| Hoxd4 | 0.7560 | 3.27E-11 | 0.4912 (0.1281) |
| Hoxd8 | 0.8454 | 1.49E-05 | N/A |
| En1 | 1.2119 | 5.18E-06 | 0.5407 (0.1045) |
| En2 | 1.6832 | 0 | 2.4048 (0.3073) |
| Nkx6-1 | 1.2110 | 5.66E-06 | 1.7457 (0.2314) |
| Otx1 | 1.1620 | 1.08E-04 | N/A |
| Otx2 | 1.4532 | 0 | 3.079 (0.3620) |
| Pou3f4 | 1.2062 | 1.35E-06 | 0.4027 (0.0994) |
| Six2 | 1.1703 | 5.04E-05 | N/A |
| UNC homeobox | 1.1689 | 2.16E-04 | N/A |
The directionality was confirmed for 11 (bolded) of 13 genes. Fold change is represented by the 2-ΔΔCP or the logarithmic change in the CP-value between trisomic and euploid samples where the level of expression of the actin control was subtracted from the level expression of the gene of interest. N/A = not assessed by qPCR. SEM for qPCR is listed in parentheses.