Table I.
Relative of abundance of selected ER proteins.
| Protein | Function | 3T31 | HeLa2 | Dendritic Cells3 |
Caco23 | Pancreatic RER4 |
|---|---|---|---|---|---|---|
| Chaperones | ||||||
| BiP=GRP78 | HSP70 family chaperone | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 |
| GRP170=Hyou1 | BiP exchange factor | 11.14 | 12.22 | 20.62 | 18.18 | 12.00 |
| Sil1=BAP | BiP exchange factor | 0.96 | 0.34 | 0.11 | 0.55 | 0.10 |
| ERdj3=DnaJB11 | J domain co-chaperone | 4.21 | 7.65 | 5.38 | 6.09 | 5.80 |
| ERdj5=DnaJC10 | J domain; reductase | 1.17 | 0.89 | 1.01 | 0.95 | 40.00 |
| P58ipk=DnaJC3 | J domain co-chaperone | 1.35 | 3.20 | 1.94 | 3.69 | |
| Sec63 | Translocon J domain | 0.37 | 9.98 | 0.61 | 6.88 | 39.60 |
| ERdj1=MTJ1=DnaJC1 | J domain co-chaperone | 0.09 | 0.47 | N.D. | 1.15 | 7.20 |
| HSP47=Serpm H1 | Collagen specific | 204.29 | 62.09 | N.D. | 81.47 | |
| GRP94 | HSP90 family chaperone | 63.44 | 90.96 | 70.65 | 123.48 | |
| CNPY3 | Client-restricted GRP94 co-chaperone | 0.94 | 2.91 | 2.85 | 4.44 | |
| Prolyl isomerases | ||||||
| Cyclophilin B | CsA-sensitive folding/ERAD facilitator | 204.00 | 165.00 | 34.00 | 208.00 | |
| FKBP13 | FK506/rapamycin binder | 10.02 | 9.57 | 7.00 | 10.96 | |
| FKBP23 | FK506/rapamycin binder | 1.74 | 1.05 | N.D. | 0.00 | |
| FKBP65 | FK506/rapamycin binder | 5.25 | 11.06 | 3.67 | ||
| Glycoprotein quality control | ||||||
| Calnexin | Membrane lectin chaperone | 19.76 | 56.75 | 17.84 | 97.65 | |
| Calreticulin | Lumenal lectin chaperone | 39.45 | 129.61 | 35.40 | 164.94 | |
| UGT1 | Glucosyl-transferase | 2.91 | 3.98 | 65.11 | 3.72 | |
| Ribophorin I | Oligosaccharyl transferase complex | 17.96 | N.D. | 14.87 | 42.70 | |
| Ribophorin II | Oligosaccharyl transferase complex |
15.23 | 24.64 | 8.54 | 16.66 | |
| Glucosidase IIα | Glycan trimming | 14.33 | 33.94 | 28.24 | ||
| Glucosidase IIβ | Glycan trimming | 9.50 | 32.74 | 4.76 | 33.61 | |
| OS-9 | ERAD lectin | N.D. | 0.31 | 0.71 | ||
| Erlectin=XTP3-B | ERAD lectin | 0.66 | 0.00 | 0.44 | ||
| Mannosidase Man1b1 | Glycan trimming | 0.18 | 0.40 | 0.31 | ||
| EDEM2 | ERAD lectin | N.D. | 0.04 | 0.06 | ||
| EDEM3 | ERAD mannosidase | N.D. | 0.28 | 0.11 | ||
| Redox proteins | ||||||
| Erol1 | Redox generation | 5.28 | 0.02 | 0.40 | 15.47 | |
| ERp44 | 5.30 | 11.06 | 5.10 | 19.42 | ||
| ERp29 | 9.37 | 31.96 | 18.75 | 47.32 | ||
| PDIA1 = PDI | Isomerase and chaperone | 78.66 | 109.76 | 27.69 | 91.24 | |
| PDIA3 = ERp57 | Glycoprotein preference | 82.51 | 70.26 | 64.81 | 91.58 | |
| PDIA4 = Erp72 | 16.09 | 28.58 | 15.01 | 43.87 | ||
| PDIA5 = Pdir | 1.16 | 1.34 | 0.13 | 3.92 | ||
| PDIA6 = P5 | 30.95 | 21.35 | 30.34 | 36.83 | ||
| Prdx4 | Redox generation | 18.74 | 31.90 | 2.35 | 6.88 | |
Data derived from published quantitative proteomic methods. The abundance of BiP/GRP78 in each dataset was set to 100 and the expression values of the other proteins were normalized to it.
1
3T3 proteomic data from [108].
3
D4-positive dendritic cells, peak intensity counts reported in [231].
2
eLa cell and Caco2 cell proteomics from [232], using peptide spectral counts. Similar results were reported by [233], based on SILAC quantitation.
4
Values from [234] and are based on purification of proteins.