Figure 3. qRT-PCR using protocols shown in figure 2D and E can detect changes in splicing induced by splicing factor knockdown.

A. C_tmax-C_t for cDNA extracted from prostate cancer (PC3) cells with lentiviral knockdown of SRPK1 or scrambled. B. Amount of VEGF calculated from standard curves in Figure 2. C. Amount of VEGF-A₁₆₅b identified by Exon 8b primers (VEGF-A₁₆₅b) or that calculated from mispriming of VEGF-A₁₆₅a. D. Proportion of VEGF that is VEGF-A₁₆₅a or VEGF-A₁₆₅b in control and knockdown cells. Values are Mean±SEM (n = 2). 3E. qPCR for VEGF-A₁₆₅a on commercially available cDNAs from 2 different companies (open bars) or cDNA reverse transcribed from freshly extracted human kidney RNA (solid bar). 3F qPCR for VEGF-A₁₆₅b on commercially available cDNAs from 2 different companies (open bars) or cDNA reverse transcribed from freshly extracted human kidney RNA (solid bar).