Table 2. Summarized quantitative values of the sarcomere structure.
| Soleus |
EDL |
||||
|---|---|---|---|---|---|
| (µm) | t/t | WT | t/t | WT | |
| Myofibril | A-band | 1.65 ± .02 | 1.64 ± .01 | 1.62 ± .01 | 1.66 ± .04 |
| M-line | 0.13 ± .001 | 0.12 ± .01 | 0.12 ± 0.01 | 0.12 ± .01 | |
| Z-line | 0.09 ± 0.01 | 0.10 ± 0.01 | 0.11 ± .01 | 0.09 ± 0.15 | |
| Section | A-band | 1.44 ± .03 | 1.48 ± .03 | 1.44 ± 0.03 | 1.49 ± .02 |
| M-line | 0.11 ± .01 | 0.12 ± .01 | 0.13 ± .01 | 0.12 ± .01 | |
| Z-line | 0.09 ± .01 | 0.09 ± .01 | 0.05 ± .01 | 0.05 ± .01 | |
Average length of A-band and width of M-line and Z-line in (t/t and WT muscles determined by negative staining (Myofibril) and thin sectioning (Section). Measurements of the electron microscopy images were averaged from approximately 10 well-oriented sarcomeres in each case. No significant differences were observed between the (t/t and WT mice in either the soleus or EDL muscles. Shorter A-band lengths in the sections compared with negatively stained myofibrils are consistent with shrinkage effects that are commonly seen during specimen preparation for thin section electron microscopy.