Figure 2. Threading of single and double-stranded DNA.

Various DNA types are compatible with molecular threading. (A) Concatamers of m13mp18 ssDNA prepared by rolling-circle amplification, extracted to 40 µm. (B) Double-stranded genomic T4 GT7 bacteriophage DNA, extracted to 40 µm. (C) Double-stranded λ bacteriophage DNA, extracted to 10 µm. All samples were threaded onto different elastomer substrates and stained with intercalating dye YOYO-1 prior to deposition.