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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Trends Cell Biol. 2013 Apr 16;23(8):357–364. doi: 10.1016/j.tcb.2013.03.001

Figure 4. Competitive Repopulation Assays.

Figure 4

(a) Marked spermatogonial stem cells of two different genotypes (red and green) are isolated from donor animals, mixed together and injected into the testes of host mouse pups lacking SSCs. Pups are raised, mated, and the progeny are analyzed for markers specific to the donor SSC populations. The proportion of each class of progeny indicates how efficiently each type of donor SSC can compete for niche access in the testis. (b) Marked hematopoietic stem cells of two different genotypes (red and green) are isolated from donor mice, mixed and injected intravenously into irradiated host mice. Contents of the bone marrow niche and the peripheral blood are analyzed to determine how efficiently each type of donor HSC can competitively engraft and produce functional blood cells.