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EDS5 localizes at the chloroplast envelope. A, CLSM images of mesophyll protoplasts from Arabidopsis expressing EDS5-YFP and the chloroplast marker RecA-CFP. B, Mesophyll protoplasts from Arabidopsis expressing EDS5-YFP transfected with the inner envelope marker prCIA5-TM2-RFP (CIA5-RFP; Cerutti et al., 1992). The protoplast isolation and transfection were carried out as described (Yoo et al., 2007). Bars = 10 μm.
