Figure 2.

Time course of major metabolite classes during pollen in vitro culture. The sum of the arbitrary units (a.u.) of all metabolites belonging to the specific class was calculated from the results matrix file. The mean ± sd of three biological replicates is shown. A, Monosaccharides and disaccharides. B, Amino acids. C, Organic acids. D, Fatty acids in percentage of total mole amounts (% mol). Black circles indicate control, and white circles indicate addition of 20 µm antimycin A after 65 min.