Table II. Functional assignment of up- and down-regulated features of plants exposed to individual and combined stress.
Transcripts found to be differentially regulated in each treatment in comparison with control plants were divided into up- and down-regulated groups and grouped according to a classification of features based on bins provided by MAPMAN. Numbers illustrate the percentage of significantly regulated features from a specific functional group relative to the percentage of features from that specific group to the entire chip. Up-regulated features have been marked as positive values and down-regulated ones as negative values. Conditions are as follows: heat (h), drought (d), TuMV (v), drought and heat (dh), TuMV and heat (vh), drought and TuMV (dv), TuMV, drought, and heat (vdh), and severe heat (severe h).
| Category | Relative Enrichment of Up-Regulated Features |
Relative Enrichment of Down-Regulated Features |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| h | d | v | vd | dh | vh | vdh | severe h | h | d | v | vd | dh | vh | vdh | severe h | |
| Photosynthesis | 0.04 | 0.00 | 0.00 | 0.09 | 0.21 | 0.14 | 0.21 | 0.31 | −7.04 | 0.00 | −8.07 | −5.87 | −5.54 | −7.73 | −5.15 | −3.56 |
| Major carbohydrates | 0.55 | 0.00 | 0.55 | 0.50 | 0.65 | 0.63 | 0.67 | 0.99 | −4.54 | −2.54 | 0.00 | −0.63 | −3.45 | −4.70 | −2.94 | −2.60 |
| Minor carbohydrates | 1.57 | 1.81 | 0.84 | 1.12 | 1.13 | 1.62 | 1.01 | 1.44 | −1.29 | 0.00 | 0.00 | −1.91 | −1.39 | −1.37 | −1.36 | −1.38 |
| Glycolysis | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.12 | 0.10 | −2.43 | 0.00 | 0.00 | −1.62 | −2.35 | −1.17 | −0.85 | −2.08 |
| Fermentation | 0.72 | 0.00 | 0.00 | 0.00 | 0.61 | 0.83 | 0.00 | 0.50 | −6.24 | 0.00 | 0.00 | 0.00 | −2.13 | −4.00 | −0.63 | −0.46 |
| Gluconeogenesis | 2.26 | 4.13 | 0.00 | 1.28 | 1.93 | 1.96 | 1.98 | 3.16 | −0.98 | 0.00 | 0.00 | 0.00 | −0.56 | 0.00 | −0.49 | 0.00 |
| Oxidative pentose phosphate pathway | 1.90 | 0.00 | 0.00 | 0.00 | 1.08 | 1.10 | 1.11 | 0.88 | −2.20 | 0.00 | 0.00 | −1.83 | −0.94 | −0.88 | −0.28 | −0.61 |
| TCA | 0.67 | 0.98 | 0.00 | 0.00 | 0.34 | 0.62 | 0.47 | 0.56 | −2.33 | 0.00 | 0.00 | 0.00 | −2.12 | −2.42 | −2.10 | −2.42 |
| ATP synthesis | 1.85 | 0.00 | 0.37 | 0.50 | 1.64 | 2.06 | 1.55 | 2.01 | −0.51 | 0.00 | 0.00 | −0.86 | −0.51 | −0.82 | −0.39 | −0.38 |
| Cell wall | 0.92 | 2.68 | 2.21 | 1.09 | 0.68 | 1.01 | 0.58 | 0.80 | −1.03 | −1.06 | −2.02 | −0.80 | −1.86 | −1.08 | −2.17 | −2.07 |
| Lipid metabolism | 0.86 | 0.81 | 0.42 | 0.82 | 1.16 | 1.06 | 1.09 | 0.89 | −1.11 | 0.00 | −0.61 | −0.96 | −1.40 | −1.16 | −1.40 | −1.72 |
| Nitrogen metabolism | 2.21 | 0.00 | 1.87 | 0.84 | 1.88 | 2.56 | 1.93 | 1.03 | 0.00 | 0.00 | 0.00 | −2.13 | −2.55 | −1.53 | −2.56 | −2.14 |
| Amino acid metabolism | 0.83 | 0.55 | 0.38 | 0.69 | 0.93 | 0.92 | 0.89 | 0.90 | −1.38 | −2.63 | 0.00 | −0.22 | −1.34 | −0.89 | −1.08 | −1.22 |
| Sulfur assimilation | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.58 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Metal handling | 1.53 | 0.86 | 2.39 | 1.34 | 0.81 | 1.78 | 0.72 | 0.58 | −3.48 | 0.00 | 0.00 | 0.00 | −2.34 | −2.63 | −2.26 | −1.83 |
| Secondary metabolism | 1.26 | 1.29 | 1.04 | 2.00 | 1.20 | 1.50 | 1.49 | 1.35 | −1.58 | −2.05 | 0.00 | −0.68 | −1.42 | −1.31 | −1.28 | −1.08 |
| Hormone metabolism | 2.09 | 3.05 | 2.34 | 2.10 | 1.67 | 1.73 | 1.33 | 1.27 | −1.60 | −3.58 | −6.80 | −3.56 | −2.28 | −1.53 | −1.93 | −1.56 |
| Cofactor | 0.00 | 0.00 | 0.00 | 0.00 | 0.19 | 0.00 | 0.20 | 0.16 | −0.78 | 0.00 | 0.00 | 0.00 | −0.67 | −0.63 | −0.79 | −0.58 |
| Tetrapyrrole synthesis | 0.22 | 0.00 | 1.13 | 0.00 | 0.19 | 0.00 | 0.00 | 0.00 | −7.35 | −15.55 | 0.00 | −11.61 | −5.95 | −6.82 | −4.84 | −4.46 |
| Stress | 1.45 | 1.38 | 1.86 | 2.03 | 1.58 | 1.50 | 1.54 | 1.20 | −1.62 | −2.07 | −0.88 | −1.32 | −1.25 | −1.60 | −1.33 | −1.32 |
| Redox regulation | 1.00 | 1.10 | 1.02 | 1.25 | 0.60 | 1.16 | 0.70 | 0.73 | −2.87 | −6.99 | 0.00 | −2.90 | −2.77 | −3.41 | −2.48 | −1.68 |
| Polyamine metabolism | 1.27 | 0.00 | 0.00 | 2.88 | 2.70 | 0.00 | 2.77 | 2.21 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | −1.10 | −0.82 |
| Nucleotide metabolism | 0.76 | 0.00 | 0.00 | 0.35 | 1.04 | 1.23 | 1.40 | 0.80 | −1.58 | −1.77 | 0.00 | −1.32 | −2.18 | −1.27 | −1.46 | −1.62 |
| Xenobiotics | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.40 | 2.21 | −0.78 | 0.00 | 0.00 | 0.00 | −0.45 | −0.63 | −0.39 | −0.87 |
| C1 metabolism | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.39 | −2.45 | 0.00 | 0.00 | 0.00 | −1.40 | −0.39 | −1.47 | −1.28 |
| Miscellaneous | 1.35 | 1.12 | 1.55 | 1.71 | 1.14 | 1.39 | 1.18 | 1.19 | −1.52 | −1.43 | −1.35 | −1.11 | −1.42 | −1.38 | −1.41 | −1.25 |
| RNA | 1.16 | 0.88 | 0.85 | 0.90 | 1.26 | 1.13 | 1.34 | 1.25 | −0.65 | −1.63 | −1.55 | −1.24 | −0.70 | −0.70 | −0.71 | −0.68 |
| DNA | 0.45 | 0.48 | 0.04 | 0.21 | 0.51 | 0.56 | 0.58 | 0.58 | −0.29 | −0.19 | −0.36 | −0.29 | −0.44 | −0.40 | −0.47 | −0.74 |
| Protein | 0.71 | 0.43 | 0.55 | 0.58 | 0.77 | 0.72 | 0.82 | 0.90 | −0.65 | −0.27 | −0.65 | −0.77 | −0.65 | −0.61 | −0.60 | −0.58 |
| Signaling | 1.12 | 2.21 | 3.40 | 2.69 | 1.05 | 0.89 | 0.66 | 0.77 | −0.75 | −0.64 | −0.41 | −0.69 | −0.93 | −0.82 | −1.21 | −1.67 |
| Cell | 0.72 | 0.66 | 0.91 | 0.85 | 0.82 | 0.68 | 0.70 | 0.71 | −0.60 | −0.35 | −0.66 | −0.52 | −0.99 | −0.81 | −0.85 | −1.34 |
| MicroRNA | 0.62 | 0.82 | 0.28 | 0.38 | 1.10 | 1.24 | 1.28 | 1.29 | −0.88 | −1.30 | −1.24 | −1.95 | −0.67 | −0.94 | −0.73 | −0.58 |
| Development | 1.45 | 0.62 | 0.94 | 1.00 | 1.34 | 1.33 | 1.56 | 1.31 | −0.79 | −0.39 | −1.12 | −0.68 | −1.05 | −1.06 | −1.14 | −0.86 |
| Transport | 1.03 | 0.64 | 1.66 | 1.51 | 1.05 | 1.09 | 1.14 | 1.29 | −1.47 | −2.79 | −0.72 | −1.45 | −1.52 | −1.36 | −1.48 | −1.29 |
| Not assigned | 1.00 | 1.10 | 0.85 | 0.85 | 0.99 | 0.97 | 0.98 | 0.98 | −0.83 | −0.70 | −0.84 | −0.82 | −0.78 | −0.83 | −0.83 | −0.85 |