. 2013 Jun 24;162(4):1911–1925. doi: 10.1104/pp.113.220152

Table I. Quantification of BRI1 and SERK3 colocalization after immunolabeling.

Comparative colocalization analysis is shown for immunolabeled BRI1-GFP and SERK3-HA in roots of 5-d-old Arabidopsis seedlings coexpressing BRI-GFP1 and SERK3-HA. BRZ (5 µm) was added to the growth medium 3 d after germination. BL (1 µm) and BFA (50 µm) were applied for 1 h. BRI1-GFP was visualized using rabbit anti-YFP/goat anti-rabbit-Alexa488 antibodies, and SERK3-HA was labeled using mouse anti-HA/goat anti-mouse-Alexa568 antibodies. Colocalizing fractions are presented as modified Manders colocalization coefficients ± se. Additionally, Pearson correlation coefficients ± se are given as independent measure for colocalization. N represents the number of regions of interest analyzed.

Treatment	BRI1-GFP	SERK3-HA	r (Pearson)	N
Plasma membrane
Untreated	0.57 ± 0.01	0.48 ± 0.02	0.14 ± 0.01	67
BRZ	0.56 ± 0.01	0.49 ± 0.03	0.12 ± 0.01	68
BRZ + BL	0.63 ± 0.02^a^,^b	0.60 ± 0.02^a^,^b	0.14 ± 0.01	86
BRZ + BFA	0.69 ± 0.02^a^,^b	0.65 ± 0.02^a^,^b	0.20 ± 0.01^a^,^b	82
BRZ + BFA/BL	0.74 ± 0.02^a^,^b	0.73 ± 0.02^a^,^b	0.24 ± 0.01^a^,^b	84
Intracellular
Untreated	0.48 ± 0.04	0.29 ± 0.03	0.07 ± 0.02	63
BRZ	0.35 ± 0.03	0.23 ± 0.03	0.06 ± 0.02	56
BRZ + BL	0.45 ± 0.04	0.37 ± 0.03^b	0.12 ± 0.02	71
BRZ + BFA	0.49 ± 0.05^b	0.41 ± 0.03^a^,^b	0.04 ± 0.02	53
BRZ + BFA/BL	0.43 ± 0.04	0.37 ± 0.03^b	0.07 ± 0.02	61
BFA compartment
BRZ + BFA	0.64 ± 0.02	0.64 ± 0.03	0.12 ± 0.02	73
BRZ + BFA/BL	0.68 ± 0.02	0.65 ± 0.03	0.13 ± 0.02	72

The mean difference is significant at P < 0.05 compared with untreated samples (two-tailed Student’s t test for equal variance). ^bThe mean difference is significant at P < 0.05 compared with BRZ-treated samples (two-tailed Student’s t test for equal variance).