Table II. Quantification of BRI1-SERK3 colocalization obtained by live root imaging.
Treatment | BRI1-GFP | SERK3-mCherry | r (Pearson) | N |
---|---|---|---|---|
Plasma membrane | ||||
Untreated | 0.73 ± 0.01 | 0.75 ± 0.01 | 0.24 ± 0.02 | 28 |
BRZ | 0.74 ± 0.01 | 0.77 ± 0.01 | 0.22 ± 0.02 | 41 |
BRZ + BL | 0.77 ± 0.0a,b | 0.81 ± 0.0a,b | 0.31 ± 0.01a,b | 47 |
BRZ + BFA | 0.89 ± 0.01a,b | 0.86 ± 0.01a,b | 0.48 ± 0.03a,b | 36 |
BRZ + BFA/BL | 0.90 ± 0.06a,b | 0.86 ± 0.01a,b | 0.59 ± 0.01a,b | 38 |
Intracellular | ||||
Untreated | 0.35 ± 0.02 | 0.55 ± 0.02 | −0.26 ± 0.01 | 184 |
BRZ | 0.41 ± 0.02a | 0.57 ± 0.02 | −0.21 ± 0.01a | 215 |
BRZ + BL | 0.46 ± 0.02a,b | 0.58 ± 0.02 | −0.16 ± 0.02a,b | 202 |
BRZ + BFA | 0.39 ± 0.02 | 0.42 ± 0.02a,b | −0.13 ± 0.01a,b | 112 |
BRZ + BFA/BL | 0.39 ± 0.02 | 0.42 ± 0.02a,b | −0.09 ± 0.03a,b | 102 |
BFA compartment | ||||
BRZ + BFA | 0.60 ± 0.02 | 0.64 ± 0.01 | −0.12 ± 0.02 | 112 |
BRZ + BFA/BL | 0.78 ± 0.01c | 0.71 ± 0.01c | 0.08 ± 0.02c | 107 |
The mean difference is significant at P < 0.05 compared with untreated samples (two-tailed Student’s t test for equal variance). bThe mean difference is significant at P < 0.05 compared with BRZ-treated samples (two-tailed Student’s t test for equal variance). cThe mean difference is significant at P < 0.05 compared with BRZ + BFA-treated samples (two-tailed Student’s t test for equal variance).