. 2013 Jun 24;162(4):1911–1925. doi: 10.1104/pp.113.220152

Table II. Quantification of BRI1-SERK3 colocalization obtained by live root imaging.

Comparative colocalization analysis is shown for live roots of 5-d-old Arabidopsis seedlings coexpressing BRI1-GFP2 and SERK3-mCherry. BRZ (5 µm) was added to the growth medium 3 d after germination. For ligand stimulation, 1 µm BL was applied for 1 h. Colocalizing fractions of BRI1-GFP and SERK3-mCherry are presented as modified Manders colocalization coefficients ± se. Additionally, Pearson correlation coefficients ± se are given. N represents the number of regions of interest analyzed. For plasma membrane analysis, one region of interest included at least three plasma membrane sections.

Treatment	BRI1-GFP	SERK3-mCherry	r (Pearson)	N
Plasma membrane
Untreated	0.73 ± 0.01	0.75 ± 0.01	0.24 ± 0.02	28
BRZ	0.74 ± 0.01	0.77 ± 0.01	0.22 ± 0.02	41
BRZ + BL	0.77 ± 0.0^a^,^b	0.81 ± 0.0^a^,^b	0.31 ± 0.01^a^,^b	47
BRZ + BFA	0.89 ± 0.01^a^,^b	0.86 ± 0.01^a^,^b	0.48 ± 0.03^a^,^b	36
BRZ + BFA/BL	0.90 ± 0.06^a^,^b	0.86 ± 0.01^a^,^b	0.59 ± 0.01^a^,^b	38
Intracellular
Untreated	0.35 ± 0.02	0.55 ± 0.02	−0.26 ± 0.01	184
BRZ	0.41 ± 0.02^a	0.57 ± 0.02	−0.21 ± 0.01^a	215
BRZ + BL	0.46 ± 0.02^a^,^b	0.58 ± 0.02	−0.16 ± 0.02^a^,^b	202
BRZ + BFA	0.39 ± 0.02	0.42 ± 0.02^a^,^b	−0.13 ± 0.01^a^,^b	112
BRZ + BFA/BL	0.39 ± 0.02	0.42 ± 0.02^a^,^b	−0.09 ± 0.03^a^,^b	102
BFA compartment
BRZ + BFA	0.60 ± 0.02	0.64 ± 0.01	−0.12 ± 0.02	112
BRZ + BFA/BL	0.78 ± 0.01^c	0.71 ± 0.01^c	0.08 ± 0.02^c	107

The mean difference is significant at P < 0.05 compared with untreated samples (two-tailed Student’s t test for equal variance). ^bThe mean difference is significant at P < 0.05 compared with BRZ-treated samples (two-tailed Student’s t test for equal variance). ^cThe mean difference is significant at P < 0.05 compared with BRZ + BFA-treated samples (two-tailed Student’s t test for equal variance).