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Al tolerance of a KO line of P. patens (PpSTOP1). A, Genomic PCR of the wild type (WT) and PpSTOP1-KO lines was conducted using primer pairs to amplify target sites (i.e. PpSTOP1 gene, NPTII gene). B, The wild-type and KO lines (KO1–KO3) were grown for 2 weeks on Al-toxic medium containing 400 μm Al at pH 4.2. Bar = 1 cm.
