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. 2013 Jun 6;162(4):1956–1964. doi: 10.1104/pp.113.216804

Figure 2.

Figure 2.

Mapping of the SSCD1 gene. The SSCD1 locus was mapped to an approximately 35-kb interval between the CAPS markers C4067 and C4102 on chromosome I (A), which is included in a single BAC F12F1 (B). Complementation assays with subcloned fragments of F12F1 (C) and the genes (D) showed that the phenotypes of sscd1-1 transgenic for the subclone ESl 6.4 (sscd1::tESl 6.4) and At1g12050 (sscd1::tSSCD1) were restored to the wild type (E). The positions shown below the markers indicate the locations of the Arabidopsis Genome Initiative map on the chromosome. The numbers in parentheses indicate the number of recombinant plants. Restriction endonucleases used to generate subclones (BSc, BamHI + SacI; KSl, KpnI + SalI; ESl, EcoRI + SalI) are shown. The fragment and gene marked in red can complement sscd1-1. The red arrows in E indicate wilted leaves.