Figure 8.

Symptom-inducing activity and in vitro processing of tengu homologs. The secreted regions of the tengu homolog from KV strain and that from PPT strain were identical at the amino-acid level. A, Transient expression assay of tengu homologs. From left to right: empty viral vector (vector), a vector expressing TENGU from OY-M, and its homologs of AY-WB, ACLR, and PPT strains. All photographs were taken at 21 dpi. Bars = 5 cm. B, In vitro processing assay of TENGU homologs. Recombinant GST fusion proteins of TENGU and its homologs were incubated with plant extract (top panel) or buffer (bottom panel). Proteins were analyzed by gel electrophoresis before (pre) and after (12 h post incubation [hpi]).