Figure 5.

Quantification of the H₂O₂ burst in N. benthamiana leaves 24 h after infiltration with either PAs or A. tumefaciens harboring the indicated constructs. Leaves were stained with DAB. H₂O₂ concentrations were quantified by xylenol orange analysis. Data are means ± sd from three independent experiments. Different letters indicate statistically significant differences (lsd; P < 0.05). A, Effect of the transient expression of CaADC1, CaADC1 K154A, or avrBsT on H₂O₂ production. B, Effect of PA treatment on H₂O₂ production. The mock control was treated with 10 mm MgCl₂. [See online article for color version of this figure.]