Figure 8.

Enhanced susceptibility of CaADC1-silenced pepper plants to avirulent Xcv (avrBsT) infection. A, Bacterial growth in empty vector control and silenced leaves. Bacterial growth in leaves was measured at 0 and 3 d after inoculation with 5 × 10⁴ cfu mL⁻¹ Xcv Ds1 (EV) and Xcv Ds1 (avrBsT). B, DAB staining and H₂O₂ quantification at different time points after inoculation with 5 × 10⁷ cfu mL⁻¹ Xcv Ds1 (EV) and Xcv Ds1 (avrBsT)_. C, NO detection and relative intensity measurement by DAF-2DA staining. D, Trypan blue staining and electrolyte leakage measurement. Control (TRV:00) and CaADC1-silenced (TRV:CaADC1) leaves were inoculated with 5 × 10⁷ cfu mL⁻¹ Xcv Ds1 (EV) and Xcv Ds1 (avrBsT). Trypan blue staining was performed 24 h after infiltration, and electrolyte leakage was monitored at the indicated time points after infiltration. Data are means ± sd from three independent experiments. Asterisks indicate statistically significant differences (Student’s t test; P < 0.05). H, Healthy leaves. [See online article for color version of this figure.]