Figure 4.

GID1a is required for after-ripening and controls RGL2 protein accumulation in the sly1 mutant background. A, GID1a is needed for after-ripening of sly1-2. Germination is shown for seeds after-ripened for 1 and 20 months incubated on MS-agar plates for 3 d at 4°C followed by 7 d at 22°C. B and C, Seeds after-ripened for 20 months were incubated on MS-moistened filter paper for 3 d at 4°C followed by 48 h at 22°C and then used to examine RGL2 protein (B) and RGL2 mRNA (C) accumulation by protein-blot and RT-PCR analyses, respectively. Total protein (60 µg) was loaded, and Ponceau staining was used as a loading control.