Figure 6.

Higher germination potential correlates with higher accumulation of FLAG-GID1b and formation of the GID1b-DELLA complex. A, Germination rates of sly1-10 (black squares) and three independent sly1-10 FLAG-GID1b seed lines (A, B, and C) sown on MS-moistened filter paper and incubated 5 d at 22°C. Under these conditions, sly1-10 does not germinate. B, A co-IP experiment performed using protein extracted from the indicated sly1-10 FLAG-GID1b seeds imbibed for 48 h at 22°C. Total protein was incubated with anti-FLAG matrix agarose. Immunoprecipitated FLAG-GID1b was detected by immunoblot with anti-FLAG, and DELLA RGA in complex with GID1b was detected with anti-RGA. Total protein (60 mg) was loaded (input). A Ponceau-stained membrane provided a loading control.