Figure 8.

Effect of GID1 overexpression on gene expression in seeds. The expression of the ABA biosynthesis gene, XERICO, GA20ox2, and XTH31 was examined by qRT-PCR. For each experiment, total was RNA extracted from seeds imbibed on MS-agar for 60 h at 4°C followed by 24 h at 22°C and then incubated for a further 12 h after the addition of a mock treatment or 10 mm GA₃. All seeds were dry after-ripened for 2 weeks, including dormant sly1-2 (D), except for sly1-2 after-ripened (AR) for 28 months For each condition, there were three biological replicates, and each replicate was subsampled three times. Error bars represent sd. Statistical significance was determined with an ANOVA using Tukey’s adjustment. P values are given in Supplemental Figure S8.