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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Neuron. 2013 Jun 27;79(2):347–360. doi: 10.1016/j.neuron.2013.05.014

Figure 2. Monosynaptic rabies virus injections into the striatum target the direct or indirect pathways.

Figure 2

Abbreviations: DS: dorsal striatum, GP: globus pallidus, EP: entopeduncular nucleus, SN: substantia nigra.

(A) Injection site and resulting fiber projections from starter cells in a D1R-Cre mouse. As expected, direct-pathway MSNs are labeled and heavily innervate EP and SNr, with some termini in GP. All scale bars indicate 250 μm.

(B) The border between dorsal striatum and globus pallidus shows that direct infection is tightly constrained to the striatum.

(C) Injection site and resulting fiber projections from starter cells in a D2R-Cre mouse. As expected, indirect-pathway MSNs heavily innervate GP, with little to no label in EP or SNr.

(D) Higher magnification at the border of striatum and globus pallidus shows strong direct infection in the striatum, with axon termini in GPe.

(E–F) Mean center and mean injection span are diagrammed with colored crosses in a sagittal section (E) and a coronal section (F). Red crosses indicate the mean extent of injections into D1R-Cre mice, and blue crosses indicate injections into D2R-Cre mice.

(G) Mean position, extent, and variability of the primary injection site for D1R-Cre and D2R-Cre mice. AP: anterior-posterior (positive values indicate anterior to bregma), LM: lateral-medial (values indicate lateral distance from bregma), DV: dorsal-ventral (negative values indicate ventral from bregma).

See also Figure S1.