Table 4. Number of sequences analyzed, observed diversity and estimated sample coverage for 16S rRNA amplicons from olives fermentations.
Reads | Observed OTUs | Shannon | Chao1 | ESC (%) | ||
Not treated olives fermentation | ||||||
Olive surfaces | ||||||
Day of fermentation | Target | |||||
3 | RNA | 1697 | 116 | 4.68 | 128.5 | 98.53 |
3 | DNA | 4258 | 74 | 2.43 | 78.33 | 99.67 |
8 | RNA | 5957 | 70 | 2.09 | 80.91 | 99.73 |
8 | DNA | 5199 | 78 | 2.92 | 101.1 | 99.58 |
90 | RNA | 1831 | 49 | 2.7 | 73 | 99.13 |
90 | DNA | 6325 | 73 | 2.79 | 88.55 | 99.70 |
Brines | ||||||
Day of fermentation | Target | |||||
8 | RNA | 1894 | 39 | 2.09 | 54.6 | 98.81 |
8 | DNA | 3980 | 56 | 2.47 | 77.38 | 99.52 |
90 | RNA | 2894 | 68 | 3.03 | 83 | 99.27 |
90 | DNA | 3763 | 77 | 3.02 | 89.05 | 99.39 |
NaOH treated olives fermentation | ||||||
Olive surfaces | ||||||
Day of fermentation | Target | |||||
3 | RNA | 1131 | 40 | 2.22 | 57.14 | 98.59 |
3 | DNA | 3306 | 81 | 2.6 | 110.08 | 99.15 |
8 | RNA | 2813 | 51 | 2 | 82.63 | 99.18 |
8 | DNA | 3171 | 53 | 2.06 | 70 | 99.43 |
90 | RNA | 2945 | 91 | 3.07 | 128.71 | 98.88 |
90 | DNA | 1465 | 80 | 3.59 | 107.19 | 97.95 |
Brines | ||||||
Day of fermentation | Target | |||||
8 | RNA | 7834 | 127 | 3.38 | 156.75 | 99.55 |
8 | DNA | 2772 | 101 | 3.56 | 125 | 98.81 |
90 | RNA | 5851 | 82 | 2.75 | 111.25 | 99.54 |
90 | DNA | 6428 | 112 | 3.27 | 137.83 | 99.52 |