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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Comp Neurol. 2013 Oct 1;521(14):10.1002/cne.23351. doi: 10.1002/cne.23351

Figure 3. DiI labels migrating neural crest in Stage 26 & 29 C. punctatum embryos.

Figure 3

Shark embryo neural tubes were vitally labeled with DiI for 24hrs. Stage 26 shark embryo shows DiI cells as two bright lines (arrows in A) and as a sheet at tail end (arrow in B). Stage 29 shark embryo shows DiI cells as two bright lines (arrows in C) and as a sheet at the tail end (arrow in magnified area in D). Pseudo-longitudinal section showed delaminated DiI cells on top of the neural tube (arrow in E) and on one portion (rostral) of the somite (arrowhead in E). (F) shows whole-mount image of the embryo after HNK1 immunostaining of lateral line placodal neurons (arrow). (G) FoxD3 antibody stain (green arrow) co-localized with DiI cells (white arrow). (H) Anti-beta III tubulin labeling (TuJ1) neurons (green arrow) along the lateral line did not overlap with DiI cells (white arrow). (I) The developing gut had a line of DiI cells (arrow). Abbreviations: NT: neural tube; D: dorsal, V: ventral, A: anterior; P: posterior.